Coding

Part:BBa_K1148012:Design

Designed by: Cécile Quéré   Group: iGEM13_Bordeaux   (2013-10-12)


4CL::STS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 259
    Illegal EcoRI site found at 2404
    Illegal EcoRI site found at 2687
    Illegal PstI site found at 2219
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 259
    Illegal EcoRI site found at 2404
    Illegal EcoRI site found at 2687
    Illegal PstI site found at 2219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 259
    Illegal EcoRI site found at 2404
    Illegal EcoRI site found at 2687
    Illegal BglII site found at 1064
    Illegal BamHI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 259
    Illegal EcoRI site found at 2404
    Illegal EcoRI site found at 2687
    Illegal PstI site found at 2219
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 259
    Illegal EcoRI site found at 2404
    Illegal EcoRI site found at 2687
    Illegal PstI site found at 2219
    Illegal AgeI site found at 921
    Illegal AgeI site found at 1030
    Illegal AgeI site found at 1156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal SapI.rc site found at 2546


Design Notes

We had to try to amplify the sequences from grape cDNA in order to avoid introns.


Source

Genomic sequence.

References